A de novo transcriptome and valid reference genes for quantitative real-time PCR in Colaphellus bowringi.

ABSTRACT:

Background

The cabbage beetle Colaphellus bowringi Baly is a serious insect pest of crucifers and undergoes reproductive diapause in soil. An understanding of the molecular mechanisms of diapause regulation, insecticide resistance, and other physiological processes is helpful for developing new management strategies for this beetle. However, the lack of genomic information and valid reference genes limits knowledge on the molecular bases of these physiological processes in this species.

Results

Using Illumina sequencing, we obtained more than 57 million sequence reads derived from C. bowringi, which were assembled into 39,390 unique sequences. A Clusters of Orthologous Groups classification was obtained for 9,048 of these sequences, covering 25 categories, and 16,951 were as

SUBMITTER: Tan QQ 

PROVIDER: S-EPMC4334893 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature