Characterization of a single mutation in TraQ in a strain of Escherichia coli partially resistant to Qβ infection.

ABSTRACT: Bacteria and virulent bacteriophages are in a prey-predator relationship. Experimental models under simplified conditions with the presence of bacteria and bacteriophages have been used to elucidate the mechanisms that have enabled both prey and predator to coexist over long periods. In experimental coevolution conducted with Escherichia coli and the virulent RNA bacteriophage Qβ in serial transfer, both coexisted for at least for 54 days, during which time they continued to change genetically and phenotypically. By day 16, an E. coli strain partially resistant to Qβ appeared and caused an approximately 10(4)-fold decrease in Qβ amplification. Whole-genome analysis of this strain suggested that a single mutation in TraQ was responsible for the partially resistant phenotype. TraQ interacts

SUBMITTER: Kashiwagi A 

PROVIDER: S-EPMC4335273 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature