Establishment of hepatitis C virus RNA-replicating cell lines possessing ribavirin-resistant phenotype.

ABSTRACT:

Background

Ribavirin (RBV) is a potential partner of interferon-based therapy and recently approved therapy using direct acting antivirals for patients with chronic hepatitis C. However, the precise mechanisms underlying RBV action against hepatitis C virus (HCV) replication are not yet understood. To clarify this point, we attempted to develop RBV-resistant cells from RBV-sensitive HCV RNA-replicating cells.

Methodology/principal findings

By repetitive RBV (100 μM) treatment (10 weeks) of 3.5-year-cultured OL8 cells, in which genome-length HCV RNA (O strain of genotype 1b) efficiently replicates, dozens of colonies that survived RBV treatment were obtained. These colonies were mixed together and further treated with high doses of RBV (up to 200 μM). By such RBV treatment, w

SUBMITTER: Satoh S 

PROVIDER: S-EPMC4336140 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature