Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking.

ABSTRACT:

Purpose

To design, fabricate, characterize, and in vivo assay clinically viable magnetic particles for MRI-based cell tracking.

Methods

Poly(lactide-co-glycolide) (PLGA) encapsulated magnetic nano and microparticles were fabricated. Multiple biologically relevant experiments were performed to assess cell viability, cellular performance, and stem cell differentiation. In vivo MRI experiments were performed to separately test cell transplantation and cell migration paradigms, as well as in vivo biodegradation.

Results

Highly magnetic nano (∼100 nm) and microparticles (∼1-2 µm) were fabricated. Magnetic cell labeling in culture occurred rapidly achieving 3-50 pg Fe/cell at 3 h for different particles types, and >100 pg Fe/cell after 10 h, without the requirement of a tra

SUBMITTER: Granot D 

PROVIDER: S-EPMC4336220 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature