Chromosome organization and replisome dynamics in Mycobacterium smegmatis.

ABSTRACT:

Unlabelled

Subcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse fluorescence microscopy for single-cell analysis of chromosome organization and DNA replisome dynamics in Mycobacterium smegmatis. We report that DNA replication takes place near midcell, where, following assembly of the replisome on the replication origin, the left and right replication forks colocalize throughout the replication cycle. From its initial position near the cell pole, a fluorescently tagged chromosomal locus (attB, 245° from the origin) moves rapidly to the replisome complex just before it is replicated.

SUBMITTER: Santi I 

PROVIDER: S-EPMC4337562 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature