Phenotypic responses of differentiated asthmatic human airway epithelial cultures to rhinovirus.

ABSTRACT:

Objectives

Human airway epithelial cells are the principal target of human rhinovirus (HRV), a common cold pathogen that triggers the majority of asthma exacerbations. The objectives of this study were 1) to evaluate an in vitro air liquid interface cultured human airway epithelial cell model for HRV infection, and 2) to identify gene expression patterns associated with asthma intrinsically and/or after HRV infection using this model.

Methods

Air-liquid interface (ALI) human airway epithelial cell cultures were prepared from 6 asthmatic and 6 non-asthmatic donors. The effects of rhinovirus RV-A16 on ALI cultures were compared. Genome-wide gene expression changes in ALI cultures following HRV infection at 24 hours post exposure were further analyzed using RNA-seq technology.

SUBMITTER: Bai J 

PROVIDER: S-EPMC4338293 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature