Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions.

ABSTRACT: In vitro evolution methodologies are powerful approaches to identify RNA with new functionalities. While Systematic Evolution of Ligands by Exponential enrichment (SELEX) is an efficient approach to generate new RNA aptamers, it is less suited for the isolation of efficient ribozymes as it does not select directly for the catalysis. In vitro compartmentalization (IVC) in aqueous droplets in emulsions allows catalytic RNAs to be selected under multiple-turnover conditions but suffers severe limitations that can be overcome using the droplet-based microfluidics workflow described in this paper. Using microfluidics, millions of genes in a library can be individually compartmentalized in highly monodisperse aqueous droplets and serial operations performed on them. This allows the different ste

SUBMITTER: Ryckelynck M 

PROVIDER: S-EPMC4338340 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature