Mouse ERG K(+) channel clones reveal differences in protein trafficking and function.

ABSTRACT:

Background

The mouse ether-a-go-go-related gene 1a (mERG1a, mKCNH2) encodes mERG K(+) channels in mouse cardiomyocytes. The mERG channels and their human analogue, hERG channels, conduct IKr. Mutations in hERG channels reduce IKr to cause congenital long-QT syndrome type 2, mostly by decreasing surface membrane expression of trafficking-deficient channels. Three cDNA sequences were originally reported for mERG channels that differ by 1 to 4 amino acid residues (mERG-London, mERG-Waterston, and mERG-Nie). We characterized these mERG channels to test the postulation that they would differ in their protein trafficking and biophysical function, based on previous findings in long-QT syndrome type 2.

Methods and results

The 3 mERG and hERG channels were expressed in HEK293 cells a

SUBMITTER: Lin EC 

PROVIDER: S-EPMC4338741 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature