The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.

ABSTRACT:

Background

The multicellular green alga Volvox carteri represents an attractive model system to study various aspects of multicellularity like cellular differentiation, morphogenesis, epithelial folding and ECM biogenesis. However, functional and molecular analyses of such processes require a wide array of molecular tools for genetic engineering. So far there are only a limited number of molecular tools available in Volvox.

Results

Here, we show that the promoter of the V. carteri nitrate reductase gene (nitA) is a powerful molecular switch for induction of transgene expression. Strong expression is triggered by simply changing the nitrogen source from ammonium to nitrate. We also show that the luciferase (g-luc) gene from the marine copepod Gaussia princeps, which previousl

SUBMITTER: von der Heyde EL 

PROVIDER: S-EPMC4339647 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature