Transhydrogenase promotes the robustness and evolvability of E. coli deficient in NADPH production.

ABSTRACT: Metabolic networks revolve around few metabolites recognized by diverse enzymes and involved in myriad reactions. Though hub metabolites are considered as stepping stones to facilitate the evolutionary expansion of biochemical pathways, changes in their production or consumption often impair cellular physiology through their system-wide connections. How does metabolism endure perturbations brought immediately by pathway modification and restore hub homeostasis in the long run? To address this question we studied laboratory evolution of pathway-engineered Escherichia coli that underproduces the redox cofactor NADPH on glucose. Literature suggests multiple possibilities to restore NADPH homeostasis. Surprisingly, genetic dissection of isolates from our twelve evolved populations revealed mer

SUBMITTER: Chou HH 

PROVIDER: S-EPMC4340650 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature