MicroRNAs regulate cell-to-cell variability of endogenous target gene expression in developing mouse thymocytes.

ABSTRACT: The development and homeostasis of multicellular organisms relies on gene regulation within individual constituent cells. Gene regulatory circuits that increase the robustness of gene expression frequently incorporate microRNAs as post-transcriptional regulators. Computational approaches, synthetic gene circuits and observations in model organisms predict that the co-regulation of microRNAs and their target mRNAs can reduce cell-to-cell variability in the expression of target genes. However, whether microRNAs directly regulate variability of endogenous gene expression remains to be tested in mammalian cells. Here we use quantitative flow cytometry to show that microRNAs impact on cell-to-cell variability of protein expression in developing mouse thymocytes. We find two distinct mechanisms

SUBMITTER: Blevins R 

PROVIDER: S-EPMC4340958 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature