Mass spectrometry-based protein identification with accurate statistical significance assignment.

ABSTRACT:

Motivation

Assigning statistical significance accurately has become increasingly important as metadata of many types, often assembled in hierarchies, are constructed and combined for further biological analyses. Statistical inaccuracy of metadata at any level may propagate to downstream analyses, undermining the validity of scientific conclusions thus drawn. From the perspective of mass spectrometry-based proteomics, even though accurate statistics for peptide identification can now be achieved, accurate protein level statistics remain challenging.

Results

We have constructed a protein ID method that combines peptide evidences of a candidate protein based on a rigorous formula derived earlier; in this formula the database P-value of every peptide is weighted, prior to the fi

SUBMITTER: Alves G 

PROVIDER: S-EPMC4341067 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature