A high-sensitivity optical device for the early monitoring of plant pathogen attack via the in vivo detection of ROS bursts.

ABSTRACT: Biotic stressors, especially pathogenic microorganisms, are rather difficult to detect. In plants, one of the earliest cellular responses following pathogen infection is the production of reactive oxygen species (ROS). In this study, a novel optical device for the early monitoring of Pseudomonas attack was developed; this device measures the ROS level via oxidation-sensitive 2', 7'-dichlorodihydrofluorescein diacetate (H2DCFDA)-mediated fluorescence, which could provide early monitoring of attacks by a range of plant pathogen; ROS bursts were detected in vivo in Arabidopsis thaliana with higher sensitivity and accuracy than those of a commercial luminescence spectrophotometer. Additionally, the DCF fluorescence truly reflected early changes in the ROS level, as indicated by an evaluation o

SUBMITTER: Zeng L 

PROVIDER: S-EPMC4341508 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature