An assessment of heavy ion irradiation mutagenesis for reverse genetics in wheat (Triticum aestivum L.).

ABSTRACT: Reverse genetic techniques harnessing mutational approaches are powerful tools that can provide substantial insight into gene function in plants. However, as compared to diploid species, reverse genetic analyses in polyploid plants such as bread wheat can present substantial challenges associated with high levels of sequence and functional similarity amongst homoeologous loci. We previously developed a high-throughput method to identify deletions of genes within a physically mutagenized wheat population. Here we describe our efforts to combine multiple homoeologous deletions of three candidate disease susceptibility genes (TaWRKY11, TaPFT1 and TaPLDß1). We were able to produce lines featuring homozygous deletions at two of the three homoeoloci for all genes, but this was dependent on the i

SUBMITTER: Fitzgerald TL 

PROVIDER: S-EPMC4342231 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature