Interferon regulatory factor 1 marks activated genes and can induce target gene expression in systemic lupus erythematosus.

ABSTRACT:

Objective

Interferon regulatory factor 1 (IRF-1) mediates both induction of interferons (IFNs) and responses to type I IFNs. It has been implicated as a critical mediator of inflammation in murine lupus models. In a previous study of chromatin modifications in monocytes from patients with systemic lupus erythematosus (SLE), IRF-1 was implicated as being associated with increased histone acetylation in this disease. The present study was undertaken to directly investigate IRF-1 binding sites on chromatin.

Methods

Cells from 9 female SLE patients and 7 female controls were examined. Monocytes were purified from peripheral blood and subjected to library preparation using a validated antibody to IRF-1. IRF-1 binding sites on chromatin were identified by chromatin immunoprecipita

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC4342285 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature