Control of human PLP1 expression through transcriptional regulatory elements and alternatively spliced exons in intron 1.

ABSTRACT: Although the myelin proteolipid protein gene (PLP1) encodes the most abundant protein in central nervous system (CNS) myelin, not much is known about the mechanisms that govern expression of the human gene (hPLP1). Much more is known about the processes that regulate Plp1 gene expression in rodents. From studies with Plp1-lacZ transgenic mice, it was determined that the first intron of mouse Plp1 (mPlp1) is required to attain high levels of expression in brain, concurrent with the active myelination period. Other studies have suggested that within mPlp1 intron 1 (>8 kb) lie several regions with enhancer-like activity. To test whether these sequences (and possibly others) in hPLP1 intron 1 are functional, deletion-transfection analysis was performed with hPLP1-lacZ constructs that contain v

SUBMITTER: Hamdan H 

PROVIDER: S-EPMC4342368 | biostudies-literature | 2015 Jan-Feb

REPOSITORIES: biostudies-literature