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Metabolic profiling of a transgenic Caenorhabditis elegans Alzheimer model.

ABSTRACT: Despite decades of research, no early-onset biomarkers are currently available for Alzheimer's disease, a cureless neurodegenerative disease afflicting millions worldwide. In this study, transgenic Caenorhabditis elegans were used to investigate changes in the metabolome after induced expression of amyloid-β. GC- and LC-MS-based platforms determined a total of 157 differential features. Some of these were identified using in-house (GC-MS) or public libraries (LC-MS), revealing changes in allantoin, cystathionine and tyrosine levels. Since C. elegans is far better suited to metabolomics studies than most other model systems, the accordance of these findings with vertebrate literature is promising and argues for further use of C. elegans as a model of human pathology in the study of AD.

SUBMITTER: Van Assche R

PROVIDER: S-EPMC4342517 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Metabolic profiling of a transgenic Caenorhabditis elegans Alzheimer model.

Van Assche Roel R Temmerman Liesbet L Dias Daniel A DA Boughton Berin B Boonen Kurt K Braeckman Bart P BP Schoofs Liliane L Roessner Ute U

Metabolomics : Official journal of the Metabolomic Society 20140730 2

Despite decades of research, no early-onset biomarkers are currently available for Alzheimer's disease, a cureless neurodegenerative disease afflicting millions worldwide. In this study, transgenic Caenorhabditis elegans were used to investigate changes in the metabolome after induced expression of amyloid-β. GC- and LC-MS-based platforms determined a total of 157 differential features. Some of these were identified using in-house (GC-MS) or public libraries (LC-MS), reveali ...[more]

PMID: 25750603

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