Molecular typing of uropathogenic Escherichia coli isolated from Korean children with urinary tract infection.

ABSTRACT:

Purpose

We investigated the molecular types of uropathogenic Escherichia coli (UPEC) by using conventional phylogrouping, multilocus sequence typing (MLST), and fimH genotyping.

Methods

Samples of patients younger than 18 years of age were collected from the Chung-Ang University Hospital over 2 years. Conventional phylogenetic grouping for UPEC strains was performed by polymerase chain reaction (PCR). Bacterial strain sequence types (STs) were classified on the basis of the results of partial sequencing of seven housekeeping genes. In addition, we analyzed nucleotide variations in a 424-base pair fragment of fimH, a major virulence factor in UPEC.

Results

Sixty-four UPEC isolates were analyzed in this study. Phylogenetic grouping revealed that group B2 was the most co

SUBMITTER: Yun KW 

PROVIDER: S-EPMC4342777 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature