Enhanced tumour cell nuclear targeting in a tumour progression model.

ABSTRACT:

Background

There is an urgent need for new approaches to deliver bioactive molecules to cancer cells efficiently and specifically.

Methods

Here we fuse the cancer cell nuclear targeting module of the Chicken Anaemia Virus Apoptin protein to the core histones H2B and H3 and utilise them in transfection, protein transduction and DNA binding assays.

Results

We found subsequent nuclear accumulation of these proteins to be 2-3 fold higher in tumour compared to normal cells in transfected isogenic human osteosarcoma and breast tumour progression models. This represents the first demonstration of enhanced nuclear targeting by Apoptin in a tumour progression model, and its functionality in a heterologous protein context. Excitingly, we found that the innate transduction abili

SUBMITTER: Nastasie MS 

PROVIDER: S-EPMC4342815 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature