Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi.

ABSTRACT:

Background

A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes. This study aimed to identify and validate a selection of reference genes for relative quantification in Talaromyces versatilis, a relevant industrial filamentous fungus. Beyond T. versatilis, this study also aimed to propose reference genes that are applicable more widely for RT-qPCR data normalization in filamentous fungi.

Results

A selection of stable, potential reference genes was carried out in silico from RNA-seq based transcriptomic data obtained from T. versatilis. A dozen functionally unrelated candidate genes were analysed by RT-qPCR assays over more than 30 relevant culture conditions. By using geNorm, we sho

SUBMITTER: Llanos A 

PROVIDER: S-EPMC4342825 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature