Nanomicroarray and multiplex next-generation sequencing for simultaneous identification and characterization of influenza viruses.

ABSTRACT: Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these metho

SUBMITTER: Zhao J 

PROVIDER: S-EPMC4344273 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature