Real-time cellular exometabolome analysis with a microfluidic-mass spectrometry platform.

ABSTRACT: To address the challenges of tracking the multitude of signaling molecules and metabolites that is the basis of biological complexity, we describe a strategy to expand the analytical techniques for dynamic systems biology. Using microfluidics, online desalting, and mass spectrometry technologies, we constructed and validated a platform well suited for sampling the cellular microenvironment with high temporal resolution. Our platform achieves success in: automated cellular stimulation and microenvironment control; reduced non-specific adsorption to polydimethylsiloxane due to surface passivation; real-time online sample collection; near real-time sample preparation for salt removal; and real-time online mass spectrometry. When compared against the benchmark of "in-culture" experiments combi

SUBMITTER: Marasco CC 

PROVIDER: S-EPMC4344306 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature