Selection of reference genes for qPCR- and ddPCR-based analyses of gene expression in Senescing Barley leaves.

ABSTRACT: Leaf senescence is a tightly regulated developmental or stress-induced process. It is accompanied by dramatic changes in cell metabolism and structure, eventually leading to the disintegration of chloroplasts, the breakdown of leaf proteins, internucleosomal fragmentation of nuclear DNA and ultimately cell death. In light of the global and intense reorganization of the senescing leaf transcriptome, measuring time-course gene expression patterns in this model is challenging due to the evident problems associated with selecting stable reference genes. We have used oligonucleotide microarray data to identify 181 genes with stable expression in the course of dark-induced senescence of barley leaf. From those genes, we selected 5 candidates and confirmed their invariant expression by both rever

SUBMITTER: Zmienko A 

PROVIDER: S-EPMC4344324 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature