Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.

ABSTRACT: We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

SUBMITTER: Ding Y 

PROVIDER: S-EPMC4344385 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature