Re-evaluation of the anticoagulant properties of high-density lipoprotein-brief report.

ABSTRACT:

Objective

This study was conducted to resolve the striking controversy between our previous report that high-density lipoprotein (HDL) enhances activated protein C (APC)/protein S anticoagulant actions and a subsequent, contradicting report that HDL lacks this activity.

Approach and results

When fresh HDL preparations from 2 laboratories were subjected to Superose 6 column chromatography, fractions containing HDL-enhanced APC:protein S anticoagulant actions in clotting assays, thereby validating our previous report. Moreover, the ability of HDL to enhance the anticoagulant actions of APC:protein S was neutralized by anti-apoAI antibodies, further indicating that the activity is because of HDL particles and not because of contaminating phospholipid vesicles. Density gradient

SUBMITTER: Fernandez JA 

PROVIDER: S-EPMC4344393 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature