Comparing viral metagenomics methods using a highly multiplexed human viral pathogens reagent.

ABSTRACT: Unbiased metagenomic sequencing holds significant potential as a diagnostic tool for the simultaneous detection of any previously genetically described viral nucleic acids in clinical samples. Viral genome sequences can also inform on likely phenotypes including drug susceptibility or neutralization serotypes. In this study, different variables of the laboratory methods often used to generate viral metagenomics libraries were compared for their abilities to detect multiple viruses and generate full genome coverage. A biological reagent consisting of 25 different human RNA and DNA viral pathogens was used to estimate the effect of filtration and nuclease digestion, DNA/RNA extraction methods, pre-amplification and the use of different library preparation kits on the detection of viral nucle

SUBMITTER: Li L 

PROVIDER: S-EPMC4344864 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature