Hyperpolarized 13C NMR detects rapid drug-induced changes in cardiac metabolism.

ABSTRACT:

Purpose

The diseased myocardium lacks metabolic flexibility and responds to stimuli differently compared with healthy hearts. Here, we report the use of hyperpolarized 13C NMR spectroscopy to detect sudden changes in cardiac metabolism in isolated, perfused rat hearts in response to adrenergic stimulation.

Methods

Metabolism of hyperpolarized [1-(13)C]pyruvate was investigated in perfused rat hearts. The hearts were stimulated in situ by isoproterenol shortly after the administration of hyperpolarized [1-(13)C]pyruvate. The hyperpolarized 13C NMR results were corroborated with 1H NMR spectroscopy of tissue extracts.

Results

Addition of isoproterenol to hearts after equilibration of hyperpolarized [1-(13)C]pyruvate into the existing lactate pool resulted in a sudden, r

SUBMITTER: Khemtong C 

PROVIDER: S-EPMC4344937 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature