A multiplex PCR assay for the detection of five influenza viruses using a dual priming oligonucleotide system.

ABSTRACT:

Background

A cost-effective, accurate and rapid simultaneous multiplex assay is required for testing and diagnoses of conventional and emerging viruses in clinical virology laboratories. We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1.

Methods

The optimized multiplex DPO PCR was used to detect 233 clinical human samples. The results were compared to those obtained with RT-qPCR, conventional PCR and immunochromatographic assay.

Results

Specificity analysis revealed that the DPO PCR assay amplified each target virus without any cross-amplification. Statistical analysis demonstrated that the multiplex DPO-PCR sensitivity was higher t

SUBMITTER: Ma X 

PROVIDER: S-EPMC4344991 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature