Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation.

ABSTRACT:

Background

Noroviruses (NoV) have become one of the most commonly reported causative agents of large outbreaks of non-bacterial acute gastroenteritis worldwide as well as sporadic gastroenteritis in the community. Currently, reverse transcriptase polymerase chain reaction (RT-PCR) assays have been implemented in NoV diagnosis, but improvements that simplify and standardize sample preparation, amplification, and detection will be further needed. The combination of automated sample preparation and real-time PCR offers such refinements.

Methods

We have designed a new real-time RT-PCR assay on the LightCycler (LC) with SYBR Green detection and melting curve analysis (Tm) to detect NoV RNA in patient stool samples. The performance of the real-time PCR assay was compared with that

SUBMITTER: Schmid M 

PROVIDER: S-EPMC434506 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature