Micro-scale and meso-scale architectural cues cooperate and compete to direct aligned tissue formation.

ABSTRACT: Tissue and biomaterial microenvironments provide architectural cues that direct important cell behaviors including cell shape, alignment, migration, and resulting tissue formation. These architectural features may be presented to cells across multiple length scales, from nanometers to millimeters in size. In this study, we examined how architectural cues at two distinctly different length scales, "micro-scale" cues on the order of ∼1-2 μm, and "meso-scale" cues several orders of magnitude larger (>100 μm), interact to direct aligned neo-tissue formation. Utilizing a micro-photopatterning (μPP) model system to precisely arrange cell-adhesive patterns, we examined the effects of substrate architecture at these length scales on human mesenchymal stem cell (hMSC) organization, gene expression,

SUBMITTER: Gilchrist CL 

PROVIDER: S-EPMC4345291 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature