Astrovirus VA1/HMO-C: an increasingly recognized neurotropic pathogen in immunocompromised patients.

ABSTRACT:

Background

An 18-month-old boy developed encephalopathy, for which extensive investigation failed to identify an etiology, 6 weeks after stem cell transplant. To exclude a potential infectious cause, we performed high-throughput RNA sequencing on brain biopsy.

Methods

RNA-Seq was performed on an Illumina Miseq, generating 20 million paired-end reads. Nonhost data were checked for similarity to known organisms using BLASTx. The full viral genome was sequenced by primer walking.

Results

We identified an astrovirus, HAstV-VA1/HMO-C-UK1(a), which was highly divergent from human astrovirus (HAstV 1-8) genotypes, but closely related to VA1/HMO-C astroviruses, including one recovered from a case of fatal encephalitis in an immunosuppressed child. The virus was detected in st

SUBMITTER: Brown JR 

PROVIDER: S-EPMC4345817 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature