Different normalization strategies might cause inconsistent variation in circulating microRNAs in patients with hepatocellular carcinoma.

ABSTRACT:

Background

Circulating microRNA (miRNA) are promising biomarkers for diagnosing and prognosticating numerous diseases. Reports have demonstrated controversial or even contradictory conclusions in studies on circulating microRNA. This study aimed to evaluate the potential bias of using different reference genes for analyzing circulating microRNAs in the same malignant digestive diseases.

Material and methods

We measured plasma concentrations of U6-snRNA, let-7a, miRNA-21, miRNA-106a, miRNA-155, miRNA-219, miRNA-221, and miRNA-16 in patients with hepatocellular carcinoma (HCC), gastric carcinoma (GC), hepatic cirrhosis, hepatitis B, and healthy volunteers using quantitative real-time polymerase chain reaction (qPCR). The GeNorm, Normfinder, BestKeeper, and Comparative ΔCq algo

SUBMITTER: Tang G 

PROVIDER: S-EPMC4345856 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature