Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.

ABSTRACT:

Background aims

Adipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yields from human subcutaneous lipoaspirates. This study reports our recent experience of isolating and immunophenotypically characterizing ASC from >60 human patients with a mean age of 43.6 and body mass index (BMI) of 27.

Methods

We examined the ASC yield per unit volume of lipoaspirate tissue, the surface antigen profile based on flow cytometry, histochemical differentiation potential along the adipogenic and osteogenic pathways, and expression of adipogenic mRNA by transcriptomic microarray and reverse transcription (RT)-polymerase chain reaction (PCR).

Results

The population (n = 64) of predominantly Caucasian (84.3%) female (90.6%) donors

SUBMITTER: Yu G 

PROVIDER: S-EPMC4346176 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature