MicroRNA-200c contributes to injury from transient focal cerebral ischemia by targeting Reelin.

ABSTRACT:

Background and purpose

MicroRNA (miR)-200c increases rapidly in the brain after transient cerebral ischemia but its role in poststroke brain injury is unclear. Reelin, a regulator of neuronal migration and synaptogenesis, is a predicted target of miR-200c. We hypothesized that miR-200c contributes to injury from transient cerebral ischemia by targeting reelin.

Methods

Brain infarct volume, neurological score and levels of miR-200c, reelin mRNA, and reelin protein were assessed in mice subjected to 1 hour of middle cerebral artery occlusion with or without intracerebroventricular infusion of miR-200c antagomir, mimic, or mismatch control. Direct targeting of reelin by miR-200c was assessed in vitro by dual luciferase assay and immunoblot.

Results

Pretreatment with miR-

SUBMITTER: Stary CM 

PROVIDER: S-EPMC4346276 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature