Characterization of primary cultures of adult human epididymis epithelial cells.

ABSTRACT:

Objective

To establish cultures of epithelial cells from all regions of the human epididymis to provide reagents for molecular approaches to functional studies of this epithelium.

Design

Experimental laboratory study.

Setting

University research institute.

Patient(s)

Epididymis from seven patients undergoing orchiectomy for suspected testicular cancer without epididymal involvement.

Intervention(s)

Human epididymis epithelial cells harvested from adult epididymis tissue.

Main outcome measure(s)

Establishment of a robust culture protocol for adult human epididymal epithelial cells.

Result(s)

Cultures of caput, corpus, and cauda epithelial cells were established from epididymis tissue of seven donors. Cells were passaged up to eight times and

SUBMITTER: Leir SH 

PROVIDER: S-EPMC4346407 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature