Adipose-derived stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the formation of microvascular networks.

ABSTRACT:

Unlabelled

Adipose-derived regenerative and stem cells, defined collectively as the stromal vascular fraction (SVF), support the formation of neovascular networks at the site of implantation. The effect of advancing age on SVF cell population effectiveness towards stimulated neovascularization was evaluated.

Methods

SVF was enzymatically isolated from adipose of young (ySVF, 4 months) or old (oSVF, 24 months) Fisher-344 rats, combined with type I collagen and polymerized. Encapsulated SVF was implanted subcutaneously into young Rag1 mice for two or four weeks. Angiogenic function of age-dependent SVF was also extensively evaluated in vitro using standard assays.

Results

In vitro studies indicated no difference in angiogenic function between ySVF and oSVF (viability, p

SUBMITTER: Aird AL 

PROVIDER: S-EPMC4346434 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature