Antibody αPEP13h reacts with lymphangioleiomyomatosis cells in lung nodules.

ABSTRACT:

Background

Lymphangioleiomyomatosis (LAM) is characterized by the proliferation in the lung, axial lymphatics (eg, lymphangioleiomyomas), and kidney (eg, angiomyolipomas) of abnormal smooth muscle-like LAM cells, which express melanoma antigens such as Pmel17/gp100 and have dysfunctional tumor suppressor tuberous sclerosis complex (TSC) genes TSC2 or TSC1. Histopathologic diagnosis of LAM in lung specimens is based on identification of the Pmel17 protein with the monoclonal antibody HMB-45.

Methods

We compared the sensitivity of HMB-45 to that of antipeptide antibody αPEP13h, which reacts with a C-terminal peptide of Pmel17. LAM lung nodules were laser-capture microdissected to identify proteins by Western blotting.

Results

HMB-45 recognized approximately 25% of LAM c

SUBMITTER: Valencia JC 

PROVIDER: S-EPMC4347535 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature