Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve.

ABSTRACT:

Purpose

Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve.

Methods

A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (R

SUBMITTER: Xie JX 

PROVIDER: S-EPMC4347977 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature