CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells: implications for the pro-inflammatory effects of this transcription factor.

ABSTRACT:

Background

The CCAAT/enhancer-binding protein β (C/EBPβ) is a transcription factor, which was first identified as a regulator of differentiation and inflammatory processes mainly in adipose tissue and liver; however, its function in the brain was largely unknown for many years. Previous studies from our laboratory indicated that C/EBPβ is implicated in inflammatory process and brain injury, since mice lacking this gene were less susceptible to kainic acid-induced injury.

Methods

We first performed cDNA microarrays analysis using hippocampal RNA isolated from C/EBPβ (+/+) and C/EBPβ (-/-) mice. Immunocytochemical and immunohistochemical studies were done to evaluate C/EBPβ and C3 levels. Transient transfection experiments were made to analyze transcriptional regulation of C3

SUBMITTER: Hernandez-Encinas E 

PROVIDER: S-EPMC4348118 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature