Angiogenic cytokines are antibody targets during graft-versus-leukemia reactions.

ABSTRACT:

Purpose

The graft-versus-leukemia (GVL) reaction is an important example of immune-mediated tumor destruction. A coordinated humoral and cellular response accomplishes leukemia cell killing, but the specific targets remain largely uncharacterized. To learn more about the antigens that elicit antibodies during GVL reactions, we analyzed patients with advanced myelodysplasia (MDS) and acute myelogenous leukemia (AML) who received an autologous, granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting tumor cell vaccine early after allogeneic hematopoietic stem cell transplantation (HSCT).

Experimental design

A combination of tumor-derived cDNA expression library screening, protein microarrays, and antigen-specific ELISAs were used to characterize sera obtained longi

SUBMITTER: Piesche M 

PROVIDER: S-EPMC4348150 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature