Recording large extracellular spikes in microchannels along many axonal sites from individual neurons.

ABSTRACT: The numerous connections between neuronal cell bodies, made by their dendrites and axons, are vital for information processing in the brain. While dendrites and synapses have been extensively studied, axons have remained elusive to a large extent. We present a novel platform to study axonal physiology and information processing based on combining an 11,011-electrode high-density complementary metal-oxide semiconductor microelectrode array with a poly(dimethylsiloxane) channel device, which isolates axons from somas and, importantly, significantly amplifies recorded axonal signals. The combination of the microelectrode array with recording and stimulation capability with the microfluidic isolation channels permitted us to study axonal signal behavior at great detail. The device, featuring t

SUBMITTER: Lewandowska MK 

PROVIDER: S-EPMC4348166 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature