Analysis of commercial kidney stone probiotic supplements.

ABSTRACT:

Objective

To examine the levels of Oxalobacter formigenes in probiotic supplements marketed by PRO-LAB, Ltd, Toronto, Canada, and capsules of Oxalo purchased from Sanzyme Ltd, Hyderabad, India, and to measure the ability of these preparations to degrade oxalate in vitro.

Methods

Probiotic supplements and pure cultures of O. formigenes were cultured in a number of media containing oxalate. Optical density at 595 nm (OD595) was used to measure bacterial growth, and ion chromatography was used to measure loss of oxalate in culture media. O. formigenes-specific and degenerate Lactobacillus primers to the oxalate decarboxylase gene (oxc) were used in polymerase chain reaction (PCR).

Results

Incubating probiotic supplements in different media did not result in the growth of

SUBMITTER: Ellis ML 

PROVIDER: S-EPMC4348557 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature