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Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry.

ABSTRACT: Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pharmacokinetic experiment by targeting a group of homologous drug transforming proteins in human hepatocytes. Our results indicate the generic applicability of this method to any biological system.

SUBMITTER: Weiß F 

PROVIDER: S-EPMC4348618 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry.

Weiß Frederik F   Schnabel Anke A   Planatscher Hannes H   van den Berg Bart H J BH   Serschnitzki Bettina B   Nuessler Andreas K AK   Thasler Wolfgang E WE   Weiss Thomas S TS   Reuss Matthias M   Stoll Dieter D   Templin Markus F MF   Joos Thomas O TO   Marcus Katrin K   Poetz Oliver O  

Scientific reports 20150304

Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pharmacokinetic experiment by targeting a group of homologous drug transforming proteins in human hepatocy  ...[more]

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