Transcriptional signature of progesterone in the fathead minnow ovary (Pimephales promelas).

ABSTRACT: A growing number of studies have examined transcriptional responses to sex steroids along the hypothalamic-pituitary-gonadal axis in teleost fishes. However, data are lacking on the molecular cascades that underlie progesterone signaling. The objective of this study was to characterize the transcriptional response in the ovary of fathead minnows (Pimephales promelas) in response to progesterone (P4). Fathead minnow ovaries were exposed in vitro to 500 ng P4/L. Germinal vesicle migration and breakdown (GVBD) was observed and microarrays were used to identify gene cascades affected by P4. Microarray analysis identified 1702 differentially expressed transcripts after P4 treatment. Functional enrichment analysis revealed that transcripts involved in the molecular functions of protein serine/th

SUBMITTER: Garcia-Reyero N 

PROVIDER: S-EPMC4349561 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature