Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.

ABSTRACT: The intracellular bacterial pathogen Legionella pneumophila provokes strong host responses and has proven to be a valuable model for the discovery of novel immunosurveillance pathways. Our previous work revealed that an environmental isolate of L. pneumophila induces a noncanonical form of cell death, leading to restriction of bacterial replication in primary mouse macrophages. Here we show that such restriction also occurs in infections with wild type clinical isolates. Importantly, we found that a lysine to arginine mutation at residue 88 (K88R) in the ribosome protein RpsL that not only confers bacterial resistance to streptomycin, but more importantly, severely attenuated the induction of host cell death and enabled L. pneumophila to replicate in primary mouse macrophages. Although con

SUBMITTER: Zhu W 

PROVIDER: S-EPMC4349785 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature