Overexpression of the transcription factor Sp1 activates the OAS-RNAse L-RIG-I pathway.

ABSTRACT: Deregulated expression of oncogenes or transcription factors such as specificity protein 1 (Sp1) is observed in many human cancers and plays a role in tumor maintenance. Paradoxically in untransformed cells, Sp1 overexpression induces late apoptosis but the early intrinsic response is poorly characterized. In the present work, we studied increased Sp1 level consequences in untransformed cells and showed that it turns on an early innate immune transcriptome. Sp1 overexpression does not activate known cellular stress pathways such as DNA damage response or endoplasmic reticulum stress, but induces the activation of the OAS-RNase L pathway and the generation of small self-RNAs, leading to the upregulation of genes of the antiviral RIG-I pathway at the transcriptional and translational levels.

SUBMITTER: Dupuis-Maurin V 

PROVIDER: S-EPMC4349862 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature