Expression and Splice Variant Analysis of Human TCF4 Transcription Factor in Esophageal Cancer.

ABSTRACT:

Objective

The human T cell transcription factor-4 (TCF4) interacts functionally with β-catenin in the Wnt signaling pathway, whose deregulation is involved in the tumorigenesis of various types of cancers. Recent studies showed that TCF4 mRNAs were subject to alternative splicing, which was proposed to be important in regulating transactivational properties of the corresponding protein isoforms. Here we investigated the splicing isoforms and the roles of TCF4 in human esophageal squamous cell carcinoma.

Methods

RT-PCR and subsequent cloning and sequencing were applied to identify the splicing isoforms. Western blotting and realtime PCR were used to analyze the expression of TCF4. Knockdown of TCF4 was achieved with siRNA and stable transfection of expression vectors was perf

SUBMITTER: He G 

PROVIDER: S-EPMC4349873 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature