Tissue specific dysregulated protein subnetworks in type 2 diabetic bladder urothelium and detrusor muscle.

ABSTRACT: Diabetes mellitus is well known to cause bladder dysfunction; however, the molecular mechanisms governing this process and the effects on individual tissue elements within the bladder are poorly understood, particularly in type 2 diabetes. A shotgun proteomics approach was applied to identify proteins differentially expressed between type 2 diabetic (TallyHo) and control (SWR/J) mice in the bladder smooth muscle and urothelium, separately. We were able to identify 1760 nonredundant proteins from the detrusor smooth muscle and 3169 nonredundant proteins from urothelium. Pathway and network analysis of significantly dysregulated proteins was conducted to investigate the molecular processes associated with diabetes. This pinpointed ERK1/2 signaling as a key regulatory node in the diabetes-ind

SUBMITTER: Tomechko SE 

PROVIDER: S-EPMC4349983 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature