Systematically ranking the tightness of membrane association for peripheral membrane proteins (PMPs).

ABSTRACT: Large-scale quantitative evaluation of the tightness of membrane association for nontransmembrane proteins is important for identifying true peripheral membrane proteins with functional significance. Herein, we simultaneously ranked more than 1000 proteins of the photosynthetic model organism Synechocystis sp. PCC 6803 for their relative tightness of membrane association using a proteomic approach. Using multiple precisely ranked and experimentally verified peripheral subunits of photosynthetic protein complexes as the landmarks, we found that proteins involved in two-component signal transduction systems and transporters are overall tightly associated with the membranes, whereas the associations of ribosomal proteins are much weaker. Moreover, we found that hypothetical proteins containin

SUBMITTER: Gao L 

PROVIDER: S-EPMC4350030 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature