Node-pore sensing enables label-free surface-marker profiling of single cells.

ABSTRACT: Flow cytometry is a ubiquitous, multiparametric method for characterizing cellular populations. However, this method can grow increasingly complex with the number of proteins that need to be screened simultaneously: spectral emission overlap of fluorophores and the subsequent need for compensation, lengthy sample preparation, and multiple control tests that need to be performed separately must all be considered. These factors lead to increased costs, and consequently, flow cytometry is performed in core facilities with a dedicated technician operating the instrument. Here, we describe a low-cost, label-free microfluidic method that can determine the phenotypic profiles of single cells. Our method employs Node-Pore Sensing to measure the transit times of cells as they interact with a series

SUBMITTER: Balakrishnan KR 

PROVIDER: S-EPMC4350414 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature